The formation of a stent-guided neuron-like network, especially under electrical stimulation, can be an attractive way to restore the function of the damaged nervous system. Hereby, Wang Hongjun and others from Stevens Institute of Technology in the United States showed that reducing the microfiber pattern coated with graphene oxide can control the formation of neuron-like networks.

Key points of this article:

1) The three-dimensional conductive stent is made based on a printed microfiber structure using near field electrostatic printing (NFEP) and graphene oxide (GO) coating. Use NFEP to obtain various microfiber patterns from poly(l-lactic acid-caprolactone) (PLCL), and adjust the fiber coverage angle (45°, 60°, 75°, 90°) and fiber diameter (15 to 148 μm) And fiber spatial organization (cobwebs and tubular structures) to achieve complexity.

2) By layer-by-layer (LbL) assembly technology, GO is coated on PLCL ultrafine fibers and reduced in situ to reduced GO (rGO). The obtained conductive scaffold with 25-50 layers of rGO exhibits excellent conductivity ( ≈0.95 S cm-1) and the ability to induce the formation of neuron-like networks along conductive microfibers under electrical stimulation (100–150 mV cm-1). Electric field (0-150 mV cm-1) and ultra-fine fiber diameter (17-150 μm) will affect neurite growth (PC-12 cells and primary mouse hippocampal neurons) and the formation of directed neuron-like networks. With the further proof of this guiding effect on neuronal cells, these conductive scaffolds may have a wide range of applications in nerve regeneration and neural engineering.

references:

Wang, J., et al., Reduced Graphene Oxide-Encapsulated Microfiber Patterns Enable Controllable Formation of Neuronal-Like Networks. Adv. Mater. 2020, 2004555.

https://doi.org/10.1002/adma.202004555

Source of information: Wonders

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