The treatment of chronic diabetic wounds remains a formidable clinical challenge, with the core difficulties being persistent inflammation, excessive oxidative stress, and impaired angiogenesis. Most existing therapies struggle to achieve the integrated coordination of real-time infection monitoring, immune modulation, and tissue repair. This article reports an inflammation-responsive DNA hydrogel that, by loading outer membrane vesicles (OMVs) derived from Lactobacillus reuteri and self-reporting probes, constructs an intelligent system capable of autonomous infection monitoring and on-demand therapeutic intervention.The hydrogel is cross-linked by Y-shaped DNA motifs and disulfide-linked chains, with conjugated indocyanine green (ICG) and black hole quencher 3 (BHQ3) on the linking chains, enabling reactive oxygen species (ROS)-triggered signal activation and controllable release of OMVs. Thanks to the inherent antioxidant and anti-apoptotic activities of OMVs, the hydrogel can not only promote keratinocyte migration, angiogenesis, and M2 macrophage polarization in vitro, but also exert efficient antibacterial effects through fluorescence-guided near-infrared photothermal therapy.In a diabetic wound model, this system significantly accelerated wound healing by enhancing collagen deposition, promoting angiogenesis, alleviating immune inflammation, and inhibiting the expression of pro-inflammatory factors. Transcriptome analysis further confirmed that it can activate tissue regeneration pathways and suppress inflammatory cascade reactions. This multifunctional platform provides a paradigm-shifting strategy for the precise management of chronic wounds.

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https://www.sciencedirect.com/science/article/abs/pii/S0168365925012088?via%3Dihub