Bone regeneration in diabetic patients remains a challenging task, mainly due to persistent oxidative stress, chronic inflammation, and impaired osteogenic potential of stem cells. Nanoenzymes have been widely used to regulate oxidative stress and inflammation and are considered promising candidates for diabetes-related bone regeneration. However, their bone-forming capacity is limited, which significantly hinders their direct application in bone repair. Here, a series of bone-inducing prussian blue (PB) nanoenzymes doped with growth factors were designed, endowing the classic antioxidant PB with bone induction capabilities. Among them, copper-doped PB (CuPB) exhibited the best performance. The selected CuPB nanoenzymes effectively eliminate various highly reactive oxygen species, inhibit inflammation, and simultaneously promote osteogenic differentiation by activating the phosphatidylinositol 3-kinase/protein kinase B (PI3K-Akt) pathway. In type 2 diabetic rats, the CuPB nanoparticle formulation and CuPB functionalized three-dimensional scaffolds significantly enhanced periodontal bone loss and bone regeneration in models of cranial bone defects. This study established an integrated "osteogenic nanoenzyme" platform that synergistically couples antioxidant and bone-inducing functions. This research was published in Advanced Materials under the title "Osteogenic Nanozymes for Diabetic Bone Regeneration via Synergistic Antioxidant and Osteoinductive Functions".
Reference Information:
DOI: 10.1002/adma.7283