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Abstract:
This study addresses the challenge of tendon-bone healing by constructing a bio-inorganic hybrid microsphere system, exploring its regulatory effect on the immune cartilage microenvironment and the underlying mechanism, and verifying its repair efficacy in tendon-bone healing. This aims to provide new experimental basis and theoretical support for the research on material regulation strategies for tendon-bone healing.
01 Research Background
Tendon-bone healing is an important research direction in the field of bone tissue engineering. The healing process is precisely regulated by the local immune cartilage microenvironment. Current repair strategies are unable to achieve efficient and precise regulation of this microenvironment and lack functional biological material systems that can be directionally regulated. Therefore, it is necessary to develop new bio-inorganic composite functional materials to promote efficient tendon-bone interface healing through regulating the immune cartilage microenvironment.
02 Main Content
This study designs and constructs a bio-inorganic hybrid microsphere system, comprehensively characterizing its physical and chemical as well as biological properties; explores the regulatory laws of this microsphere on the immune cartilage microenvironment at the tendon-bone healing site, and analyzes the cellular and molecular-level mechanisms of the regulatory process; verifies the promoting effect of the microsphere system on the immune cartilage microenvironment and tendon-bone healing through in vitro and in vivo experiments, and clarifies the intrinsic correlation between material characteristics, microenvironment regulation, and tendon-bone repair.
03 Research Design
1. Complete the design and preparation of the bio-inorganic hybrid microsphere system, and comprehensively characterize its basic characteristics such as morphology, structure, composition, and biocompatibility;2. Conduct in vitro cell experiments to explore the influence of the microsphere on the biological behaviors of immune-related cells, chondrocytes, and tendon-bone repair-related cells;3. Establish a tendon-bone injury animal model and conduct in vivo experiments to verify the regulatory effect of the microsphere system on the immune cartilage microenvironment at the injury site and the repair status of tendon-bone tissue;4. From the molecular pathway level, deeply analyze the core mechanism of the microsphere system regulating the immune cartilage microenvironment and promoting tendon-bone healing.
04 Results
The constructed bio-inorganic hybrid microsphere system has good physical and chemical properties and biocompatibility, and can effectively regulate the cellular composition and cytokine expression profile of the immune cartilage microenvironment at the tendon-bone healing site, optimize the local immune response and cartilage matrix remodeling process, improve the histological structure of the tendon-bone healing interface, and achieve functional repair of the tendon-bone interface; at the same time, it clarifies the key cellular and molecular mechanisms of the microsphere system regulating the immune cartilage microenvironment and promoting tendon-bone healing.
05 Extension of Thoughts
Based on the material design ideas of this study, the component ratio and structure morphology of the bio-inorganic hybrid microsphere can be further optimized to enhance its precise regulatory ability of the immune cartilage microenvironment; the regulatory strategy of this microsphere can be extended to other microenvironment regulation studies in tissue repair fields to enrich the design ideas of functional biological materials; and the fine molecular mechanism of the interaction between bio-inorganic materials and the immune cartilage microenvironment can be further explored to provide more comprehensive theoretical support for the rational design of microenvironment-regulating functional biological materials in bone tissue engineering.
