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Metal-free hydrogen-bonded organic frameworks (HOFs) are porous materials formed by hydrogen bonds between organic components. They possess excellent biocompatibility and enzyme compatibility in biomedical applications. However, the fabrication of multi-enzyme cascading antioxidant nanoenzymes using HOFs for the treatment of cerebral ischemia-reperfusion injury (CIRI) remains challenging. Here, selenium-containing nanohydrogen-bonded organic frameworks (SeHOFs) were synthesized as glutathione peroxidase mimics, in situ encapsulating superoxide dismutase (SOD) and catalase (CAT), to form a mixed cascading antioxidant system (SeHOF@CAT@SOD). SeHOFs exhibit enhanced catalytic activity, maintain enzyme functionality, and can protect them from temperature, proteolysis, and denaturation. Metal-free nanoenzymes demonstrate excellent biocompatibility and cascading catalytic efficacy, capable of eliminating reactive oxygen species and reducing cell apoptosis and iron depletion in vitro. Peptide modification enhances the accumulation at the infarcted site, effectively reducing the infarct volume, oxidative stress, neuronal apoptosis, iron depletion, and inflammation in the CIRI model. This study highlights the potential of nanohydrogen-bonded organic frameworks as a scaffold for the development of advanced therapeutic nanoenzymes in ischemic stroke treatment. This research was published in ACS Nano under the title "Selenium-Containing Nanoscale Hydrogen-Bonded Organic Framework Nanozymes for Multienzyme Cascade Antioxidant-Targeted Therapy of Cerebral Ischemia–Reperfusion Injury".
References:
DOI: 10.1021/acsnano.5c20015
