This magnetic ball is based on polymer modified silicon-hydroxyl magnetic ball, and then through chemical coupling reaction, streptomyces affinity


Functionalized streptavidin magnetic balls prepared by modifying polymer magnetic balls


  


1. Schematic diagram of the structure of the magnetic ball


The structure diagram of DiscoverMagTM-SiO2 Magnetic Beads is shown in Figure 1.


  


  


  


Figure 1 Schematic diagram of the structure of DiscoverMagTM-SiO2 Magnetic Beads


  


  


2. Function description


The DiscoverMagTM TM-SiO2 Magnetic Beads prepared by our company are a good DNA extractor.


Take the magnetic beads.


When using silico-hydroxy magnetic beads to extract nucleic acids, high concentrations of chaotropic salts (NaI, NaClO4, GuHCl,


GuSCN, etc.). The high concentration of salt ions can effectively shield the electrostatic repulsion in the solution. At the same time, the chaotropic salt ions also


It can competitively combine with the surface of magnetic beads and nucleic acid molecules, destroy the original hydration layer on the surface of both, and promote the magnetic beads.


Adsorption of nucleic acid molecules on the surface (hydrogen bond + van der Waals force). Due to the commonly used chaotropic salts such as guanidine ion pair PCR


It has an inhibitory effect, so they must be removed in the subsequent washing steps. The removal effect can be measured by the absorbance ratio.


To monitor.


3. References


1. Hutchens, T. W.; Porath, J. O. Protein recognition of immobilized ligands:


promotion of selective adsorption. Clinical Chemistry. 1987, 33 (9): 1502–8 [2013-04-


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