Protein purification magnetic beads are filled with a magnetic inner core inside agarose microspheres, and metal ions Ni2 or Co2, Ni2 or Co2, GSH are immobilized on the surface, which can form a coordination with histidine or GST fusion protein in the protein His tag Bond to bind the target protein to the magnetic beads, and then separate it by a magnetic separator.
Basis for analysis of purification results: electrophoresis diagram, buffer formulation and protein properties
It is recommended that users run SDS-PAGE gel electrophoresis with crude protein solution, flow-through solution (the remaining liquid on which the protein is bound to the magnetic beads), washing solution, and eluent to analyze the experimental results.
Nickel purification magnetic beads and GSH purification magnetic beads are a new type of functional material designed for the effective and rapid purification of histidine-tagged protein and GST fusion protein. They can be purified directly from biological samples in one step by magnetic separation. The target protein significantly simplifies the purification process and reduces equipment requirements. It is suitable for the rapid and convenient purification of histidine-tagged protein in scientific research and industrial fields.