It is an AuPtCo ternary alloy nanozyme and is applied to chemiluminescence bioanalysis. Polyhedral nanozymes (AuPtCo NPs) with Au, Pt, Co ternary alloys were prepared by a one-step thermal reduction method. The synthesized AuPtCo NPs have both peroxidase-like and catalase-like catalytic activities, and can catalyze the isoluminol (ABEI)-H2O2 system to generate a "flash"-type chemiluminescence signal.