Methacrylylated carboxymethyl chitosan (CMCSMA)

Storage and transportation
Dry state; -20C protected from light. 6 months. Can be transported at room temperature. Solution: Protect from light at 4°C for 7 days; Protect from light at -20°C for 3 months; Repeated freezing and thawing of the solution will affect the product performance, please prepare it for immediate use.
Effective date
See packaging for production date.


Solution preparation
1 Prepare 0.25% (w/v) initiator standard solution
(1) Take 10ml of PBS and add it to a brown bottle filled with initiator LAР {contains 0.025g LAP); (2) Heat and dissolve in a water bath at 40-50°C for 15 minutes, shaking several times during the period.
The LAP standard solution can be stored for 12 months at 4°C and protected from light. 2 Preparation of CMCSMA solution (1) Take the required quality of CMCSMA into the centrifuge tube;
(2 Take the required volume of initiator standard solution and add it to the above centrifuge tube;
(3) Dissolve at 25-50°C for 1-2 hours with constant stirring/shaking during the period; (CMCSMA has high viscosity and longer dissolution time) (4) Sterilize the CMCSMA solution with a 0.22urm sterile syringe filter.
Two-dimensional cell culture recommendations
>Pour the CMCSMA solution into the well plate;
(96-well plate: 50-100uL/well, 46-well plate: 100-300L/well, 24-well plate: 300~500uL/well) Use a 405nm light source and irradiate for 10-30 seconds to gel. It can be lighted Time and intensity control the gel strength; add culture medium to the well to cover the gel, place it in a 37°C incubator for 5 minutes to clean the auspicious product, and aspirate the culture medium;> just add the cell suspension to the well plate. Perform operations such as medium replacement, observation and photography according to the experimental design
(No special requirements for operating procedures).
Three-dimensional cell culture recommendations
> Collect cells and resuspend them with CMCSMA solution to prepare cell suspension; add cell suspension to the well plate;
(96-well plate: 50~100L/well, 48-well plate: 100~30QL/well, 24-well plate: 300~500uL/well L) with 405nm light source. Irradiate for 10-30 seconds to gel. The intensity of the gel can be adjusted by the light time and intensity; Tip: Add one hole to solidify one hole to prevent cell precipitation.
>Add culture medium to each well, in a 37°C incubator for 5 minutes, wash the sample and remove the culture medium;
Add fresh medium and cultivate for a long time. Perform operations such as culture medium replacement, observation and photographing, immunofluorescence staining, etc. according to the experimental design (no special requirements for operating procedures).
Reminder: Do not look directly at the curing light source.

Warm tip: the products supplied by Beijing Beike Xincai Technology Co., Ltd. are only used for scientific research, not for human body