已传文件：photo/1773121782.png
This study investigated a composite scaffold material used for repairing severe size bone defects. The research was conducted by researchers Fang Lei, He Min, Zhang Tao, et al. To address the challenge of the disorderly connection between inflammation, angiogenesis and bone formation during the healing process of large bone defects, an innovative three-dimensional radial-arranged nanofiber composite scaffold loaded with bone marrow-derived mesenchymal stromal cells (BMSCs) was constructed. This scaffold guides cell infiltration through its radial structure, and the GelMA hydrogel ensures the survival and residence of the loaded cells to prolong their paracrine effect time. The hydroxyapatite (HAp) component enhances the paracrine output of BMSCs while providing bone conduction cues, thereby achieving sequential regulation from immune modulation to angiogenesis and ultimately promoting bone regeneration at the damaged site. The related research results were published in the authoritative journal of biomaterials, "Bioactive Materials". Innovation Points
01 Sequential regulation design based on the synergy of structure and composition
This research does not solely focus on the physical structure or chemical composition of the scaffold, but ingeniously combines the two to meet the biological requirements of different stages of bone regeneration. The radially arranged fiber structure guides cells to migrate inward rapidly in the early stage; the GelMA hydrogel provides shelter for the loaded BMSCs, protecting them from premature immune clearance and extending the "window period" for their paracrine function; while the HAp component continuously provides bone-inducing signals in the later stage, and through material-cell interaction enhances the paracrine function of BMSCs earlier, achieving temporal coordination of the regeneration process.
02 Dual guarantee strategy for cell survival and function
To address the bottleneck of low survival rate and rapid decline of paracrine activity of MSCs after transplantation due to the inflammatory microenvironment, this scaffold provides dual guarantees. Firstly, the encapsulation of GelMA hydrogel creates a relatively mild three-dimensional microenvironment for BMSCs, improving their survival rate and retention time. More importantly, the HAp component not only serves as a bone-conducting material but is also found to be able to upregulate the paracrine output of BMSCs, especially by influencing cell metabolism and activating key signaling pathways, enhancing their regenerative capacity both in "quantity" and "quality".
03 In-depth study on the mechanism of promoting regeneration based on metabolic reprogramming
This study delves deeply into the potential mechanism of material regulation of cell behavior, discovering that the composite scaffold can upregulate the glycerophospholipid metabolism of BMSCs to support early proliferation and activate the PI3K/Akt signaling pathway to drive their differentiation towards osteogenic direction. This mechanism revealed at the metabolic and signal transduction levels provides a new perspective for understanding how materials "educate" cells and achieve the best regenerative efficacy.
Journal Name: Bioactive Materials
Publication Date: March 6, 2026
DOI: 10.1016/j.bioactmat.2026.02.059 R & D Team: Lei Fang, Min He, Tao Zhang, Bowen Gong, Li Ruan, Jichuan Qiu, Jiajia Xue, Feng Tian
