[Product Introduction]
The magnetic bead method plasmid DNA mass extraction kit is a kind of bacterial plasmid DNA mass extraction kit recently developed by Suzhou Beike Zhenze Biotechnology Co., LTD. This product combines magnetic nanoseparation technology with SDS alkali lysis of bacterial cells to extract high quality plasmid DNA from recombinant E. coli cultures in a simple, fast and low-cost way. The cell fragments and SDS complex precipitate under centrifugal force. Under certain conditions, the magnetic beads can adsorb plasmid DNA in the supernatant well, and other impurities such as proteins and salt ions can be removed by washing. When conditions change, the beads release the adsorbed plasmid DNA. Up to 1.5 mg of plasmid DNA can be recovered from 100-200 mL Luria Broth (LB) culture in 45 minutes. Note that the actual yield and optimal culture volume depend on the plasmid and medium. The plasmids extracted by this kit are of high purity, and the OD260/OD280 ratio is generally between 1.7 and 1.9, and the OD260/OD230 ratio is greater than 2.0, which can be directly used for subsequent experiments such as transformation, DNA sequencing, PCR, PCAR-based mutation, in vitro transcription and enzyme digestion.
【 characteristics 】
L For high-copy plasmids, more than 1.5 mg plasmid DNA was obtained from 200 mL overnight culture (OD600 = 2.0);
L Compared with similar products, the extraction efficiency is higher, the purity of the obtained plasmid is higher;
L Compared with the column method, the centrifugation times can be greatly reduced, and the plasmid with better integrity can be obtained.
Multiple samples can be processed simultaneously to realize high-pass quantification and automation of plasmid extraction.
[Preservation methods and Precautions]
L Please store the Beads and RNase A in the kit at 2-8℃ and the rest of the reagents at room temperature. Please see the packing for the expiry date.
L Freezing and centrifugation are strictly prohibited. Freezing and centrifugation may cause irreversible damage to magnetic beads.
L Magnetic beads will gather into clusters after long-term placement, thus reducing the surface area of magnetic beads and reducing the recovery rate of samples. It is necessary to thoroughly mix magnetic beads by eddy oscillation before use (usually 20 seconds of eddy oscillation).
[Kit composition]
component
5 times
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Resuspension solution
100 ml
2-8 ℃
Lysis buffer
100 ml
2-8 ℃
Neutralization solution
100 ml
2-8 ℃
Wash buffer
75% ethanol (for users)
2-8 ℃
Elution buffer
100 ml
2-8 ℃
PuriMag bead
10 ml
2-8 ℃
RNase A
10 ml
2-8 ℃
Isopropyl alcohol
Users should bring along their own
The normal temperature
DNA concentration and purity detection :(1) the size of the obtained genomic DNA fragments was related to factors such as sample preservation time and shear force during operation. The concentration and purity of the obtained DNA fragments could be detected by agarose gel electrophoresis and ultraviolet spectrophotometer. (2) DNA should have a significant absorption peak at OD260, and OD260=1 is equivalent to about 50ng/ul double-stranded DNA and 40ng/ul single-stranded DNA. The OD260/OD280 ratio should be 1.7~1.9. If deionized water is used in elution, the ratio will be low, because pH and ions will affect the absorbance, which does not mean low purity.
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Warm tip: the products supplied by Beijing Beike Xincai Technology Co., Ltd. are only used for scientific research, not for human body |
| Item ID |
CAS |
ID |
Pack |
Parameter |
Stock |
Make up |
Price |
| BK2021081223 |
|
BK2021081223 |
|
|
100 |
|
$0 |
